題目：Using fruit flies to understand sleep regulation and function
演講人：Jeffrey Donlea, Ph.D.
Neurobiology Department at UCLA
Sleep-promoting neurons in the dorsal fan-shaped body (dFB) of Drosophila are vital for sleep homeostasis, but how these cells impose sleep on the organism is not well understood. We report that dFB neurons communicate via inhibitory transmitters, including allatostatin-A (AstA), with interneurons connecting the superior arch with the ellipsoid body of the central complex. Using a combination of behavioral genetics, electrophysiology, and optogenetics, we have begun to outline a homeostatic negative feedback circuit that regulates sleep and arousal in the fruit fly. By virtue of this autoregulatory loop, dFB-mediated inhibition interrupts processes that incur a sleep debt, allowing restorative sleep to rebalance the books.